Ciro Santilli
OurBigBook.comCiro Santilli believes that molecular biology technologies will be a large part of the next big things as shown at: Section "Molecular biology technologies".
Bibliography:
- www.youtube.com/watch?v=mS563_Teges&list=PLQbPquAyEw4dQ3zOLrdS1eF_KJJbUUyBx Biophysical Techniques Course 2022 by the MRC Laboratory of Molecular Biology. Holy crap that playlist is a tour de force of molecular biology techniques in 2022!
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Study of the metabolome.
Some notable examples:
For a commented initial example, see: e. Coli K-12 MG1655 gene thrA.
KEGG does the visual maps well.
But BioCyc is generally better otherwise.
TODO vs KEGG.
TODO human presumably?
I don't think it has any advantage over KEGG however, besides historical interest? Maybe slightly more manual layout and so more beautiful?
James Somers (rightly) likes to point to it as a "biology is awesome" thing.
An artificial metabolic pathway using synthetic biology technology.
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Design software for synthetic biological circuit.
The input is in Verilog! Overkill?
Then it essentially maps to a standard cell library of biological primitives!
You label cells with isotopes rather than fluorescent substances. Vendors claim that this allows much wider N-way sorts, e.g. 2022 Fluidigm claims around 40/50, because the fluorecent spectrum is too wide to do much more than 7/8 way splits.
You modify the DNA of a cell and stick a fluorescent protein right before or after another protein. Then when it gets translated, the GFP is stuck to the protein of interest, which hopefully hasn't lost its function as a result, then you can just see the protein of interest.
Using green fluorescent protein as a protein tag.
The 3D structure of GFP is so cool. It is so clearly a bottle with a fluorescent bit well isolated right in the middle. Like a little lamp.
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Technique widely used to measure the size of DNA strands, most often PCR output of a region of interest.
A simple sample application is gel electrophoresis alelle determination.
In the case of indel mutations (see limits of gel electrophoresis for minimal size difference issues), it is possible to determine the allele with gel electrophoresis. You can just read out the alleles right in the gel. It is a thing of beauty.
As of 2020, this method appears to be much cheaper than DNA sequencing approaches.
- 1982 www.pnas.org/doi/pdf/10.1073/pnas.80.6.1579 DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels by Fischer and Lerman (1982). It is possible then.
Biologists are obsessed with these!
These can be used to break cells apart from tissue, and also break up larger DNA or RNA molecules into smaller ones, suitable for sequencing.
The author Horace Freeland Judson was a MacArthur fellow, no wonder he found the time to write this bible!
On the Internet Archive Open Library:
Max Delbrück is quoted as saying:Nice way to put it.
So in retrospect what the denouement was, was that both the principle of replication and the principle of readout are DNA very simple, and the actual machinery for doing it is immensely complex. That's the way it has turned out."
When Thomas F. Anderson had started taking and publishing the first phage electron microscope images:
Now, Anderson later wrote, "We could really see the phage as tadpole shaped particles, whose heads ranged from 600 to 800 A [...] Anderson wrote. "I remember particularly the reaction of Alfred Hershey's teacher, kindly old Professor J. J. Bronfenbrenner, who had worked on bacteriophages for many years at Washington University in St. Louis. ... When he first saw our pictures ... he clapped the palm of his hand to his forehead and exclaimed, 'Mein Gott! They've got tails!'"
Nice quote from Pauling's
The requirements are stringent ones. [...]. In order that the principles of modem structural chemistry may be applied with the power that their reliability justifies, molecular models must be constructed with great accuracy. For example, molecular models on the scale of 2.5 cm 1 angstrom unit, have to be made with a precision better than 0.01 cm.
The requirements are stringent ones. [...]. In order that the principles of modem structural chemistry may be applied with the power that their reliability justifies, molecular models must be constructed with great accuracy. For example, molecular models on the scale of 2.5 cm 1 angstrom unit, have to be made with a precision better than 0.01 cm.
Ancestors
Incoming links
- Bert Hubert
- Caroline Ulbricht
- Deep tech
- James Somers
- Molecular biology technologies
- Molecular Sciences Course of the University of São Paulo
- Omics
- Physics and the illusion of life
- Sponsor Ciro Santilli's work on OurBigBook.com
- Why you should give money to Ciro Santilli
- Taxonomic rank
- The Google Story
- Whole cell simulation