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Video 1. The Scanning Electron Microscope by MaterialsScience2000 (2014) Source. Shows operation of the microscope really well. Seems too easy, there must have been some extra setup before however. Impressed by how fast the image update, it is basically instantaneous. Produced by Prof. Dr.-Ing. Rainer Schwab from the Karlsruhe University of Applied Sciences.
It sees and moves individual atoms!!!
This technique has managed to determine protein 3D structures for proteins that people were not able to crystallize for X-ray crystallography.
It is said however that cryoEM is even fiddlier than X-ray crystallography, so it is mostly attempted if crystallization attempts fail.
By looking at Figure 1. "A cryoEM image", you can easily understand the basics of cryoEM.
We just put a gazillion copies of our molecule of interest in a solution, and then image all of them in the frozen water.
Each one of them appears in the image in a random rotated view, so given enough of those point of view images, we can deduce the entire 3D structure of the molecule.
Ciro Santilli once watched a talk by Richard Henderson about cryoEM circa 2020, where he mentioned that he witnessed some students in the 1980's going to Germany, and coming into contact with early cryoEM. And when they came back, they just told their principal investigator: "I'm going to drop my PhD theme and focus exclusively on cryoEM". That's how hot the cryo thing was! So cool.
Figure 1. A cryoEM image. Source. This is the type of image that you get out of a raw CryoEM experiment.
Video 2. Two Photon Microscopy by Nemonic NeuroNex (2019) Source. Shows a prototype of a two-photon electron microscope on an optical table, and describes it in good detail, well done.
One of its main applications is to determine the 3D structure of proteins.
Sometimes you are not able to crystallize the proteins however, and the method cannot be used.
Crystallizing is not simple because:
  • you need a considerable amount of the protein
  • sometimes it only crystallizes if you add some extra small chemical that stabilizes it
cryogenic electron microscopy can sometimes determine the structures of proteins that failed crystallization.
Often used as a synonym for X-ray crystallography, or to refer more specifically to the diffraction part of the experiment (exluding therefore sample preparation and data processing).
cyclotrons produce the better images, but they are expensive/you have to move to them and order a timeslot.
Lab-based just use some X-ray source from the lab, so it is much move convenient e.g. for a pharmaceutical company doing a bunch of images. The Wikipedia image shows such a self-contained lab system:
Crystallography determination with a transmission electron microscopy instead of the more classical X-ray crystallography.
As of 2022, this channel is still finding its feet. But it has promise.
Unfortunately it does not show sample preparation, and it does not use controlled cultures, so we are never sure which species are represented.
The channel is also notable for the fact that the author makes his own music.
Video 3. Behind the Scenes by Sci-Inspi (2020) Source. His name is Manuael, aka Manu, and he is the chemistry lab technician at a community college.