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One of its main applications is to determine the 3D structure of proteins.
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Sometimes you are not able to crystallize the proteins however, and the method cannot be used.
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Crystallizing is not simple because:
  • you need a considerable amount of the protein
  • sometimes it only crystallizes if you add some extra small chemical that stabilizes it
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cryogenic electron microscopy can sometimes determine the structures of proteins that failed crystallization.
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Ancestors

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