Ciro Santilli
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DNA sequencing
"DNA"
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wiki
Big excitement picture at:
molecular biology technologies
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A concrete experiment has been done at
Section 6. "Sequencing"
on section
sequencing
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Table of contents
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Base calling
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"DNA sequencing"
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DNA microarray
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"DNA sequencing"
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Metagenomics
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"DNA sequencing"
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Short-read DNA sequencing
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"DNA sequencing"
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Long-read DNA sequencing
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"Short-read DNA sequencing"
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RNA-Seq
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"DNA sequencing"
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Gene expression profiling
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"RNA-Seq"
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DNA sequencing company
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"DNA sequencing"
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Illumina
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"DNA sequencing company"
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Bridge amplification
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"Illumina"
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Solexa
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"Illumina"
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Oxford Nanopore Technologies
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"DNA sequencing company"
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Oxford Nanopore Technologies product
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"Oxford Nanopore Technologies"
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PromethION
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"Oxford Nanopore Technologies product"
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Oxford Nanopore MinION
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"Oxford Nanopore Technologies product"
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How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
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"Oxford Nanopore MinION"
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1. Experiment background
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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2. Overview of the experiment
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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2.1. Why Oxford Nanopore was used instead of Illumina for the sequencing
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"Overview of the experiment"
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3. Sample collection
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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4. DNA extraction
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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4.1. Filtration with vacuum pump
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"DNA extraction"
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4.2. Post filtration purification
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"DNA extraction"
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5. PCR
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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5.1. PCR verification with gel electrophoresis
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"PCR"
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6. Sequencing
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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6.1. Pre-sequencing preparation
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"Sequencing"
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6.2. Using the Oxford Nanopore
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"Sequencing"
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7. Bioinformatics
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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8. Conclusions
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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9. Protocols used
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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9.1. Qiagen DNeasy PowerWater Kit
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"Protocols used"
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9.2. Qiagen QIAquick PCR Purification Kit
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9.3. Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit
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10. Equipment used
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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10.1. Thermo Scientific Nalgene Polysulfone Reusable Bottle Top Filters
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"Equipment used"
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10.2. KNF Laboport series laboratory vacuum pump
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10.3. Scientific Industries Inc. Vortex-Genie 2
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10.4. VWR Micro Star 17 microcentrifuge
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"Equipment used"
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10.5. VELP Scientifica WIZARD IR Infrared Vortex Mixer
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10.6. Marshal Scientific MJ Research PTC-200 Thermal Cycler
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10.7. GE MagRack 6
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10.8. BTLab Systems Mini Centrifuge
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10.9. Fisher Scientific UVP LM-26E Benchtop 2UV Transilluminator
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10.10. Biochrom SimpliNano spectrophotometer
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11. External links to this page
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"How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it"
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Tagged
Sequencing
Ancestors
DNA
Molecular biology
Level of organization of bodies
Biology
Natural science
Science
Ciro Santilli's Homepage
Incoming links
DNA detection
DNA microarray
European Tower of Babel
Molecular biology technologies
Overview of the experiment
Sequence alignment
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