Ciro Santilli $$ Sponsor Ciro $$ 中国独裁统治 China Dictatorship 新疆改造中心、六四事件、法轮功、郝海东、709大抓捕、2015巴拿马文件 邓家贵、低端人口、西藏骚乱
As of 2018, Ciro Santilli believes that this could be the next big thing in biology technology.
"De novo" means "starting from scratch", that is: you type the desired sequence into a computer, and the synthesize it.
The "de novo" part is important, because it distinguishes this from the already well solved problem of duplicating DNA from an existing DNA template, which is what all our cells do daily, and which can already be done very efficiently in vitro with polymerase chain reaction.
Many startup companies are attempting to create more efficient de novo synthesis methods:
Notably, the dream of most of those companies is to have a machine that sits on a lab bench, which synthesises whatever you want.
TODO current de novo synthesis costs/time to delivery after ordering a custom sequence.
The initial main applications are likely going to be:
but the real pipe dream is building and bootstraping entire artificial chromosomes
News coverage:
Video 6. Nuclera eDNA enzymatic de novo DNA synthesis explanatory animation (2021) Source. The video shows nicely how Nuclera's enzymatic DNA synthesis works:
  • they provide blocked nucleotides of a single type
  • add them with the enzyme. They use a werid DNA polymerase called terminal deoxynucleotidyl transferase that adds a base at a time to a single stranded DNA strand rather than copying from a template
  • wash everything
  • do deblocking reaction
  • and then repeat until done