Ciro Santilli
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Also known as: Quantitative PCR (qPCR).
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Like PCR, but the amplification machine measures the concentration of DNA at each step.
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This describes one possible concentration detection method with fluorescent molecules that only become fluorescent when the DNA is double stranded (SYBR Green)
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Video 131. "Polymerase Chain Reaction (PCR) - Quantitative PCR (qPCR)" by Applied Biological Materials - abm (2016) Source.
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This allows you to predict the exact initial concentration by extrapolating the exponential curve backwards.
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TODO: vs non-real-time PCR. Why can't you just divide by 2 for every heating step to reach back the original concentration? Likely the reaction reach saturation at an unknown step.
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TODO: vs non-real-time PCR in medical diagnostics: do you really need to know concentration for diagnostics? Isn't it enough to know if the virus is present or not?
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