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Oxford Nanopore Technologies product
...
Level of organization of bodies
Molecular biology
DNA
DNA sequencing
DNA sequencing company
Oxford Nanopore Technologies
OurBigBook.com
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articles: 33
Table of contents
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PromethION
Oxford Nanopore Technologies product
Oxford Nanopore MinION
Oxford Nanopore Technologies product
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31
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
Oxford Nanopore MinION
1k
30
1.
Experiment background
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
2.
Overview of the experiment
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
1
2.1.
Why Oxford Nanopore was used instead of Illumina for the sequencing
Overview of the experiment
3.
Sample collection
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
4.
DNA extraction
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
2
4.1.
Filtration with vacuum pump
DNA extraction
4.2.
Post filtration purification
DNA extraction
5.
PCR
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
1
5.1.
PCR verification with gel electrophoresis
PCR
6.
Sequencing
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
2
6.1.
Pre-sequencing preparation
Sequencing
6.2.
Using the Oxford Nanopore
Sequencing
7.
Bioinformatics
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
8.
Conclusions
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
9.
Protocols used
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
3
9.1.
Qiagen DNeasy PowerWater Kit
Protocols used
9.2.
Qiagen QIAquick PCR Purification Kit
Protocols used
9.3.
Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit
Protocols used
10.
Equipment used
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
10
10.1.
Thermo Scientific Nalgene Polysulfone Reusable Bottle Top Filters
Equipment used
10.2.
KNF Laboport series laboratory vacuum pump
Equipment used
10.3.
Scientific Industries Inc. Vortex-Genie 2
Equipment used
10.4.
VWR Micro Star 17 microcentrifuge
Equipment used
10.5.
VELP Scientifica WIZARD IR Infrared Vortex Mixer
Equipment used
10.6.
Marshal Scientific MJ Research PTC-200 Thermal Cycler
Equipment used
10.7.
GE MagRack 6
Equipment used
10.8.
BTLab Systems Mini Centrifuge
Equipment used
10.9.
Fisher Scientific UVP LM-26E Benchtop 2UV Transilluminator
Equipment used
10.10.
Biochrom SimpliNano spectrophotometer
Equipment used
11.
External links to this page
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
Ancestors
(10)
Oxford Nanopore Technologies
DNA sequencing company
DNA sequencing
DNA
Molecular biology
Level of organization of bodies
Biology
Natural science
Science
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