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One cool thing we did in this procedure was to use magnetic separation with magnetic beads to further concentrate the DNA: Figure 32. "GE MagRack 6 pipetting.".
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The beads are coated to stick to the DNA, which allows us to easily extract the DNA from the rest of the solution. This is cool, but bio people are borderline obsessed by those beads! Go figure!
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Figure 32. GE MagRack 6 pipetting. Source.
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Figure 33. GE MagRack 6 eppendorf with magnetic beads mounted. Source.
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Then we prepared the DNA for sequencing with the Oxford Nanopore specific part: Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit.
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Here some of the steps required a bit more of vortexing for mixing the reagents, and for this we used the VELP Scientifica WIZARD IR Infrared Vortex Mixer which appears to be quicker to use and not as strong as the Vortex Genie 2 previously used to break up the cells:
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Figure 34. VELP Scientifica WIZARD IR Infrared Vortex Mixer running. Source.
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After all that was done, the DNA of the several 400 ml water bottles and hours of hard purification labour were contained in one single Eppendorf!
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Ancestors