Ciro Santilli OurBigBook.com $£ Sponsor €¥ 中国独裁统治 China Dictatorship 新疆改造中心、六四事件、法轮功、郝海东、709大抓捕、2015巴拿马文件 邓家贵、低端人口、西藏骚乱
One cool thing we did in this procedure was to use magnetic separation with magnetic beads to further concentrate the DNA: Figure 32. "GE MagRack 6 pipetting.".
The beads are coated to stick to the DNA, which allows us to easily extract the DNA from the rest of the solution. This is cool, but bio people are borderline obsessed by those beads! Go figure!
Figure 32. GE MagRack 6 pipetting. Source.
Figure 33. GE MagRack 6 eppendorf with magnetic beads mounted. Source.
Then we prepared the DNA for sequencing with the Oxford Nanopore specific part: Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit.
Here some of the steps required a bit more of vortexing for mixing the reagents, and for this we used the VELP Scientifica WIZARD IR Infrared Vortex Mixer which appears to be quicker to use and not as strong as the Vortex Genie 2 previously used to break up the cells:
Figure 34. VELP Scientifica WIZARD IR Infrared Vortex Mixer running. Source.
After all that was done, the DNA of the several 400 ml water bottles and hours of hard purification labour were contained in one single Eppendorf!

Ancestors

  1. Sequencing
  2. How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
  3. Oxford Nanopore MinION
  4. Oxford Nanopore Technologies product
  5. Oxford Nanopore Technologies
  6. DNA sequencing company
  7. DNA sequencing
  8. DNA
  9. Molecular biology
  10. Level of organization of bodies
  11. Biology
  12. Natural science
  13. Science
  14. Ciro Santilli's Homepage