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The first thing we did was to filter the water samples with a membrane filter that is so fine that not even bacteria can pass through, but water can.
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Therefore, after filtration, we would have all particles such as bacteria and larger dirt pieces in the filter.
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From the 1 liter in each bottle, we only used 400 ml because previous experiments showed that filtering the remaining 600 ml is very time consuming because the membrane filter gets clogged up.
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Therefore, the filtration step allows us to reduce those 400 ml volumes to more manageable Eppendorf tube volumes: Figure 13. "An Eppendorf tube.". Reagents are expensive, and lab bench centrifuges are small!
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Figure 13. An Eppendorf tube. Source. They are small, convenient and disposable.
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Figure 14. Labelled Eppendorf tubes on a rack. Source.
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Since the filter is so fine, filtering by gravity alone would take forever, and so we used a vacuum pump to speed thing up!
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Figure 15. Peeling the vacuum pump filter protection peel before usage. Source.
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Figure 16. Placing the vacuum pump filter. Source.
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Video 2. Pouring the water sample into the vacuum tube and turning on the vacuum pump. Source.
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