Ciro Santilli  Sponsor 中国独裁统治 China Dictatorship 新疆改造中心、六四事件、法轮功、郝海东、709大抓捕、2015巴拿马文件 邓家贵、低端人口、西藏骚乱
The first thing we did was to filter the water samples with a membrane filter that is so fine that not even bacteria can pass through, but water can.
Therefore, after filtration, we would have all particles such as bacteria and larger dirt pieces in the filter.
From the 1 liter in each bottle, we only used 400 ml because previous experiments showed that filtering the remaining 600 ml is very time consuming because the membrane filter gets clogged up.
Therefore, the filtration step allows us to reduce those 400 ml volumes to more manageable Eppendorf tube volumes: Figure 13. "An Eppendorf tube". Reagents are expensive, and lab bench centrifuges are small!
Figure 13. . Source. They are small, convenient and disposable.
Figure 14.
Labelled Eppendorf tubes on a rack
. Source.
Since the filter is so fine, filtering by gravity alone would take forever, and so we used a vacuum pump to speed thing up!
For that we used:
Figure 15.
Peeling the vacuum pump filter protection peel before usage
. Source.
Figure 16.
Placing the vacuum pump filter
. Source.
Video 2.
Pouring the water sample into the vacuum tube and turning on the vacuum pump
. Source.


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